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Your Position: Home > Immune Checkpoint Proteins > LAG-3 > LA3-H525c

Human LAG-3 / CD223 Protein, Llama IgG2b Fc Tag, low endotoxin (MALS verified)

  • Synonym
    LAG3,CD223,FDC
  • Source
    Human LAG-3, Llama IgG2b Fc Tag (LA3-H525c) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    Online(Leu 23 - Leu 450) P18627-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 74.0 kDa. The protein migrates as 90 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LAG-3, Llama IgG2b Fc Tag (Cat. No. LA3-H525c) SDS-PAGE gel

Human LAG-3, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human LAG-3, Llama IgG2b Fc Tag (Cat. No. ) MALS images

The purity of Human LAG-3, Llama IgG2b Fc Tag (Cat. No. LA3-H525c) was more than 90% and the molecular weight of this protein is around 170-190 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human LAG-3, Llama IgG2b Fc TagHuman LAG-3, Llama IgG2b Fc Tag (Cat. No. LA3-H525c) ELISA bioactivity

Immobilized Human LAG-3, Llama IgG2b Fc Tag (Cat. No. LA3-H525c) at 2 μg/mL (100 μL/well) can bind Anti-Human LAG3 MAb with a linear range of 0.2-4 ng/mL (QC tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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