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Your Position: Home > All Other Proteins > TNFSF11 > RAL-H82F9

Biotinylated Human TNFSF11 / RANKL / CD254 Protein, Avitag™,Fc Tag

  • Synonym
    RANKL,CD254,TRANCE,OPGL,ODF
  • Source
    Biotinylated Human TNFSF11, Avitag,Fc Tag (RAL-H82F9) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).
    Predicted N-terminus: Gly
  • Molecular Characterization
    Online(Gly 64 - Asp 245) AAC51762.1

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

    The protein has a calculated MW of 49.2 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human TNFSF11, Avitag,Fc Tag (Cat. No. RAL-H82F9) SDS-PAGE gel

Biotinylated Human TNFSF11, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human TNFSF11, Avitag,Fc TagBiotinylated Human TNFSF11, Avitag,Fc Tag (Cat. No. RAL-H82F9) ELISA bioactivity

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Avitag,Fc Tag (Cat. No. RAL-H82F9) with a linear range of 0.4-13 ng/mL (QC tested).

Biotinylated Human TNFSF11, Avitag,Fc TagBiotinylated Human TNFSF11, Avitag,Fc Tag (Cat. No. RAL-H82F9) ELISA bioactivity

Immobilized Human OPG, Fc Tag (Cat. No. TNB-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Avitag,Fc Tag (Cat. No. RAL-H82F9) with a linear range of 0.3-10 ng/mL (Routinely tested).

  • Background
    Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:18 Details
  • Latest Research Phase:Approved

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