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Your Position: Home > Cytokines > IL-17A & IL-17F > ILF-H82W1

Biotinylated Human IL-17A&IL-17F Protein, Avitag™,His Tag

  • Synonym
    IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F
  • Source
    Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (ILF-H82W1) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)).
    Predicted N-terminus: Gly 24
  • Molecular Characterization
    Online(Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F)) Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)

    Biotinylated Human IL-17A&IL-17F, Avitag,His Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 34.1 kDa. Subunit IL-17A&IL-17F is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The reducing (R) protein migrates as 40-43 kDa due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) SDS-PAGE gel

Biotinylated Human IL-17A&IL-17F, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human IL-17A&IL-17F, Avitag,His TagBiotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) ELISA bioactivity

Immobilized Anti-Human IL17A MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) with a linear range of 0.2-6 ng/mL (QC tested).

  • Background
    Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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