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Your Position: Home > All Other Proteins > CEACAM-5 > CE5-H5226

Human CEACAM-5 / CD66e Protein, His Tag (MALS verified)

  • Synonym
    CEACAM-5,CD66e,CEA,Meconium antigen 100
  • Source
    Human CEACAM-5, His Tag (CE5-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731).
    Predicted N-terminus: Lys 35
  • Molecular Characterization
    Online(Lys 35 - Ala 685) P06731

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 72.2 kDa. The protein migrates as 100-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CEACAM-5, His Tag (Cat. No. CE5-H5226) SDS-PAGE gel

Human CEACAM-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CEACAM-5, His Tag (Cat. No. ) MALS images

The purity of Human CEACAM-5, His Tag (Cat. No. CE5-H5226) was more than 95% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CEACAM-5, His TagHuman CEACAM-5, His Tag (Cat. No. CE5-H5226) ELISA bioactivity

Immobilized Human CEACAM-5, His Tag (Cat. No. CE5-H5226) at 1 μg/mL (100 μL/well) can bind Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Human CEACAM-5, His TagHuman CEACAM-5, His Tag (Cat. No. CE5-H5226) ELISA bioactivity

Immobilized Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 Antibody at 1 μg/mL (100 μL/well) can bind Human CEACAM-5, His Tag (Cat. No. CE5-H5226) with a linear range of 10-39 ng/mL (Routinely tested).

  • Background
    Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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