This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 40 kDa, 32 kDa and 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Glypican 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human Glypican 3, His Tag(Cat. No. GP3-H52H4) is more than 90% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.
Anti-human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3, His Tag (Cat. No. GP3-H52H4) with an affinity constant of 1.33 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3, His Tag (Cat. No. GP3-H52H4) with an affinity constant of 1.48 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human Glypican 3, His Tag (Cat. No. GP3-H52H4) and negative control protein respectively, washed and then followed by FITC anti-his tag antibody and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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