This protein carries no "tag".
The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-15, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-15, Tag Free (Cat. No. IL5-H4117) at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) with a linear range of 0.6-39 ng/mL (QC tested).
Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, Tag Free (Cat. No. IL5-H4117) with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (QC tested).
Loaded Human IL-2 R beta, Fc Tag (SPR verified) (Cat. No. ILB-H5253) on Protein A Biosensor, can bind Human IL-15, Tag Free (Cat. No. IL5-H4117) with an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human IL-15, Tag Free (Cat. No. IL5-H4117) stimulates proliferation of CTLL-2 cells. The ED50 for this effect is 0.097-0.19 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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