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Your Position: Home > Anti-idiotypic Antibody > Cetuximab > Anti-Cetuximab Antibodies

Anti-Cetuximab Antibodies

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Cetuximab F(ab')2.
  • Isotype
    IgG1/kappa
  • Specificity
    Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 years under sterile conditions after reconstitution.

SDS-PAGE
Anti-Cetuximab Antibodies (Cat. No. CEB-Y28) SDS-PAGE gel

Anti-Cetuximab Antibodies on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Anti-Cetuximab AntibodiesAnti-Cetuximab Antibodies (Cat. No. CEB-Y28) ELISA bioactivity

Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized cetuximab at 1 µg/ml, add increasing concentrations of Anti-Cetuximab Antibodies (Cat. No. CEB-Y28, 10% human serum) and then add biotinylated cetuximab at 5 µg/ml. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 2.4 ng/mL.

Anti-Cetuximab AntibodiesAnti-Cetuximab Antibodies (Cat. No. CEB-Y28) ELISA bioactivity

Demonstration of the specificity of Anti-Cetuximab Antibodies (Cat. No. CEB-Y28) to the cetuximab.

Anti-Cetuximab AntibodiesAnti-Cetuximab Antibodies (Cat. No. CEB-Y28) ELISA bioactivity

Measured by its neutralizing ability in a functional ELISA. Immobilized cetuximab at 0.2 μg/mL (100 μL/well) can bind pre-mixed Anti-Cetuximab Antibodies (Cat. No. CEB-Y28) and Biotinylated EGFRvIII (Cat. No. EGR-H82E0) with an inhibition rate of 100%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Cetuximab Antibodies (mouse IgG1, Cat. No. CEB-Y28) captured on CM5 chip via anti-mouse antibodies surface, can bind Human cetuximab with an affinity constant of 1.51 pM.

  • Background
    Cetuximab is an epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $300.00

Price(USD) : $1980.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

Datasheet & Documentation

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