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Your Position: Home > Cytokines > IL-15 > IL5-H82F3

Biotinylated Human IL-15 Protein, Fc,Avitag™ (MALS verified)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    IL-15,Interleukin-15,MGC9721
  • Source
    Biotinylated Human IL-15, Fc,Avitag (IL5-H82F3) is expressed from human 293 cells (HEK293). It contains AA Asn 49 - Ser 162 (Accession # P40933-1).
    Predicted N-terminus: Asn 49
  • Molecular Characterization
    Online(Asn 49 - Ser 162) P40933-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 41.0 kDa. The protein migrates as 54-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-15, Fc,Avitag (Cat. No. IL5-H82F3) SDS-PAGE gel

Biotinylated Human IL-15, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human IL-15, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human IL-15, Fc,Avitag(Cat. No. IL5-H82F3) is more than 90% and the molecular weight of this protein is around 85-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human IL-15, Fc,AvitagBiotinylated Human IL-15, Fc,Avitag (Cat. No. IL5-H82F3) ELISA bioactivity

Immobilized Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15, Fc,Avitag (Cat. No. IL5-H82F3) with a linear range of 0.5-8 ng/mL (QC tested).

  • Background
    Interleukin 15 is also known as IL15, IL-15, and is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. Interleukin 15 (IL-15) regulates T and natural killer (NK) cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). IL-15 has been shown to enhance the anti-tumor immunity of CD8+ T cells in pre-clinical models. A phase I clinical trial to evaluate the safety, dosing, and anti-tumor efficacy of IL-15 in patients with metastatic melanoma and renal cell carcinoma (kidney cancer) has begun to enroll patients at the National Institutes of Health.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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