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Your Position: Home > Non SARS-Cov-2 Kits > CD47 > EP-102

CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair

For research use only.
  • Background
    Immune checkpoint pathway is a focal point of today’s cancer research. CD47 is one of the best characterized checkpoint proteins. CD47 binding to SIRP alpha aids in tumor evasion of the immune system. Therefore, the pharmaceutical inhibition of CD47 has been considered a promising strategy by many oncologists.
  • Application
    This pair is useful for screening for inhibitors of human CD47 binding to human SIRP alpha.
    It is for research use only.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store all items at -70℃. After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable after storage at: Room temperature (RT) for 1 month in lyophilized state; -20℃ for 5 year in lyophilized state; -70℃ for 6 months under sterile conditions after reconstitution.
  • Assay Principles
    This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new CD47 pathway inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized human CD47 and in-house developed biotinylated SIRP alpha protein. This product is uniquely suitable for rapid high-throughput screening of putative CD47 inhibitors. Briefly, we provide you with a biotinylated human SIRP alpha protein, a human CD47 protein, an anti-CD47 neutralizing antibody (as method verified Reference), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    1. Coat the plate with human CD47.

    2. Mix biotinylated human SIRP alpha and your molecule of interest.

    3. Add to the coated human CD47.

    4. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the ability of your compound to inhibit CD47: SIRP alpha binding will be determined by comparing OD readings among different experimental groups.

Typical Data
CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay PairCD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair (Cat. No. EP-102) ELISA bioactivity

CD47: SIRP ALPHA [BIOTINYLATED] BINDING IN THE ABSENCE OF INHIBITORS
Immobilized human CD47 protein at 3 μg/mL (100 μL/well) can bind biotinylated human SIRP alpha with a linear range of 0.004-0.125 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting (QC tested).

CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay PairCD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair (Cat. No. EP-102) ELISA bioactivity

INHIBITION OF CD47: SIRP ALPHA [BIOTINYLATED] BINDING BY ANTI-CD47 NEUTRALIZING ANTIBODY
Serial dilutions of anti-CD47 neutralizing antibody (Catalog # CD7-NA002) (1:2 serial dilutions, from 10 μg/mL to 0.04 μg/mL or 66.67 nM to 0.26 nM) was added into CD47: biotinylated SIRP alpha binding reactions. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $600.00

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  • Number of Drugs in Clinical Trials:36 Details
  • Latest Research Phase:Phase 3 Clinical

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