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CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair

For research use only.
Materials Provided
IDComponentsSize (96 tests)Size (480 tests)
A004-214Human CD4735 μg160 μg
A005-214Biotinylated Human SIRP alpha10 μg10 μg
A003-214Streptavidin-HRP10 μg10 μg
CD7-NA002Anti-CD47 Neutralizing Antibody20 μg100 μg
  • Background
    Immune checkpoint pathway is a focal point of today’s cancer research. CD47 is one of the best characterized checkpoint proteins. CD47 binding to SIRP alpha aids in tumor evasion of the immune system. Therefore, the pharmaceutical inhibition of CD47 has been considered a promising strategy by many oncologists.
  • Application

    This pair is useful for screening for inhibitors of human CD47 binding to human SIRP alpha.

  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store all items at -70℃.

    After reconstitution, the stock solution should be kept at -70℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable after storage at:

    • Room temperature (RT) for 1 month in lyophilized state;

    • -20℃ for 1 year in lyophilized state;

    • -70℃ for 6 months under sterile conditions after reconstitution.

  • Assay Principles

    This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new CD47 pathway inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized human CD47 and in-house developed biotinylated SIRP alpha protein. This product is uniquely suitable for rapid high-throughput screening of putative CD47 inhibitors. Briefly, we provide you with a biotinylated human SIRP alpha protein, a human CD47 protein, an anti-CD47 neutralizing antibody (as method verified Reference), and Streptavidin-HRP reagent. Your experiment will include 4 simple steps:

    • a) Coat the plate with human CD47.

    • b) Mix biotinylated human SIRP alpha and your molecule of interest.

    • c) Add to the coated human CD47.

    • d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the ability of your compound to inhibit CD47: SIRP alpha binding will be determined by comparing OD readings among different experimental groups.

Typical Data

Binding of Biotinylated Human SIRP alpha to Immobilized Human CD47 in a Functional ELISA Assay.

Immobilized human CD47 protein at 3 μg/mL (100 μL/well) can bind biotinylated human SIRP alpha with a linear range of 0.004-0.125 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points

Inhibition of CD47: SIRP alpha [Biotinylated] Binding by Anti-CD47 Neutralizing Antibody.

Serial dilutions of anti-CD47 neutralizing antibody (Catalog # CD7-NA002) (1:2 serial dilutions, from 10 μg/mL to 0.04 μg/mL) were added into CD47: biotinylated SIRP alpha binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.

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Price(USD) : $600.00



  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:20 Details
  • Latest Research Phase:NDA/BLA


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