A-Z> Inhibitory Kit > CD47 > CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair | Catalog | Components | Size (96 tests) | Size (480 tests) |
|---|---|---|---|
| A004-214 | Human CD47 | 35 μg | 160 μg |
| A005-214 | Biotinylated Human SIRP alpha | 10 μg | 10 μg |
| A003-214 | Streptavidin-HRP | 10 μg | 10 μg |
| CD7-NA002 | Anti-CD47 Neutralizing Antibody | 20 μg | 100 μg |
This pair is useful for screening for inhibitors of human CD47 binding to human SIRP alpha.
Upon receipt, please store all items at -70℃.
After reconstitution, the stock solution should be kept at -70℃.
It is recommended not to freeze thaw more than 3 times.
No activity loss is observed after storage at:
Room temperature (RT) for 1 month in lyophilized state;
-20℃ for 1 year in lyophilized state;
-70℃ for 6 months under sterile conditions after reconstitution.
This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of new CD47 pathway inhibitors. This assay employs a simple colorimetric ELISA platform, which measures the binding between immobilized human CD47 and in-house developed biotinylated SIRP alpha protein. This product is uniquely suitable for rapid high-throughput screening of putative CD47 inhibitors. Briefly, we provide you with a biotinylated human SIRP alpha protein, a human CD47 protein, an anti-CD47 neutralizing antibody (as method verified Reference), and Streptavidin-HRP reagent. Your experiment will include 4 simple steps:
a) Coat the plate with human CD47.
b) Mix biotinylated human SIRP alpha and your molecule of interest.
c) Add to the coated human CD47.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit CD47: SIRP alpha binding will be determined by comparing OD readings among different experimental groups.
Binding of Biotinylated Human SIRP alpha to Immobilized Human CD47 in a Functional ELISA Assay.
Immobilized human CD47 protein at 3 μg/mL (100 μL/well) can bind biotinylated human SIRP alpha with a linear range of 0.004-0.125 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points
Inhibition of CD47: SIRP alpha [Biotinylated] Binding by Anti-CD47 Neutralizing Antibody.
Serial dilutions of anti-CD47 neutralizing antibody (Catalog # CD7-NA002) (1:2 serial dilutions, from 10 μg/mL to 0.04 μg/mL) were added into CD47: biotinylated SIRP alpha binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.
Price(USD) : $600.00
Price(USD) : $2150.00
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