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Your Position: Home > Cytokines > Complement Component C5a > C5A-M51H4

Mouse Complement Component C5a Protein, His Tag

  • Synonym
    C5a,Complement Component 5a
  • Source
    Mouse C5a, His Tag (C5A-M51H4) is expressed from E.coli cells. It contains AA Asn 679 - Arg 755 (Accession # P06684).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Asn 679 - Arg 755) P06684

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 10.9 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse C5a, His Tag (Cat. No. C5A-M51H4) SDS-PAGE gel

Mouse C5a, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse C5a, His TagMouse C5a, His Tag (Cat. No. C5A-M51H4) ELISA bioactivity

Immobilized Mouse C5a, His Tag (Cat. No. C5A-M51H4) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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