>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Cetuximab Antibodies (recommended for ADA assay) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized cetuximab at 1 µg/mL, add increasing concentrations of Anti-Cetuximab Antibodies (Cat. No. CEB-Y27, 10% human serum) and then add biotinylated cetuximab at 2 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 1.2 ng/mL.
Demonstration of the specificity of Anti-Cetuximab Antibodies (Cat. No. CEB-Y27) to the cetuximab.
Measured by its neutrlizing ability in a functional ELISA. Immobilized cetuximab at 0.2 μg/mL (100 μL/well) can bind pre-mixed Anti-Cetuximab Antibodies (Cat. No. CEB-Y27) and Biotinylated EGFRvIII (Cat. No. EGR-H82E0) with a inhibition rate of 100%.
Anti-Cetuximab Antibodies (mouse IgG1, Cat. No. CEB-Y27) captured on CM5 chip via anti-mouse antibodies surface, can bind Human cetuximab with an affinity constant of 7.28 pM.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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