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Your Position: Home > Protein > IL-15 R alpha > ILA-H82F4

Biotinylated Human IL-15 R alpha / CD215 Protein, Fc,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

  • Synonym
    IL-15 R alpha,CD215,IL15RA,IL-15RA,IL-15R-alpha
  • Source
    Biotinylated Human IL-15 R alpha, Fc,Avitag (ILA-H82F4) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Thr 205 (Accession # Q13261-1).
    Predicted N-terminus: Ile 31
  • Molecular Characterization
    Online(Ile 31 - Thr 205) Q13261-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 46.6 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) SDS-PAGE gel

Biotinylated Human IL-15 R alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human IL-15 R alpha, Fc,AvitagBiotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) ELISA bioactivity

Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) with a linear range of 2-39 ng/mL (QC tested).

Biotinylated Human IL-15 R alpha, Fc,AvitagBiotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) ELISA bioactivity

Immobilized Mouse IL-15, Fc Tag (Cat. No. IL5-M5255) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) with a linear range of 10-156 ng/mL (Routinely tested).

  • Background
    IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL-15RA, also known as CD215, IL-15 R alpha, is a widely expressed 60 kDa transmembrane glycoprotein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. An interleukin (IL)-15 superagonist/IL-15 receptor α fusion complex (IL-15SA/IL-15RA; ALT-803) activates the IL-15 receptor on CD8 T cells and NK cells, and has shown significant anti-tumor. Signal transduction involves SYK. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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