>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Detection of cetuximab by bridging ELISA in serum. Immobilized Anti-Cetuximab Antibodies (Cat. No. CEB-Y29) at 2 μg/mL, add increasing concentrations of cetuximab (10% human serum) and then add Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 6 ng/mL.
Immobilized cetuximab at 0.1 μg/mL (100 μL/well) can bind Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31) with a linear range of 0.39-25 ng/mL.
ELISA analysis shows that the binding of cetuximab to Human EGF R, His Tag (Cat. No. EGR-H5222) was inhibited by increasing concentration of Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31). The concentration of cetuximab used is 3 ng/mL.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed a series of homogeneous CD3 proteins with good bioactivity. They are verified as 1:1 heterodimers by nonreductive electrophoresis and MALS as well.
ACRO screened a few different beads and bring this tightly controlled superparamagnetic microspheres to you. This product can be widely applied for immunocapture, bio panning and flow cytometry.
ACROBiosystems has developed a series of kit products using its high-quality SARS-CoV-2 antigen proteins to improve the efficiency of anti-SARS-CoV-2 drugs and vaccines development.
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