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Your Position: Home > Antibodies > Cetuximab > Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD)

Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD)

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Cetuximab F(ab')2.
  • Isotype
    IgG1/kappa
  • Specificity
    Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.
  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (13.5 angstroms) is used in this product to minimize potential steric hindrance.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1-3 as determined by the HABA assay.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 years under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD) (Cat. No. CEB-BY31) SDS-PAGE gel

Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD)Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD) (Cat. No. CEB-BY31) ELISA bioactivity

Detection of cetuximab by bridging ELISA in serum. Immobilized Anti-Cetuximab Antibodies (Cat. No. CEB-Y29) at 2 μg/mL, add increasing concentrations of cetuximab (10% human serum) and then add Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 6 ng/mL.

Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD)Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD) (Cat. No. CEB-BY31) ELISA bioactivity

Immobilized cetuximab at 0.1 μg/mL (100 μL/well) can bind Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31) with a linear range of 0.39-25 ng/mL.

Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD)Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD) (Cat. No. CEB-BY31) ELISA bioactivity

ELISA analysis shows that the binding of cetuximab to Human EGF R, His Tag (Cat. No. EGR-H5222) was inhibited by increasing concentration of Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31). The concentration of cetuximab used is 3 ng/mL.

  • Background
    Cetuximab is an epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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