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Your Position: Home > Cytokines > HGF > HGF-H52H3

Human HGF Protein, His Tag (MALS verified)

  • Synonym
    HGF,HPTA,SF
  • Source
    Human HGF, His Tag (HGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).
    Predicted N-terminus: Gln 32
  • Molecular Characterization
    Online(Gln 32 - Ser 728) P14210-1

    This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90kDa (alpha & Beta chain), 58-63 (alpha chain), 33 kDa (Beta chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human HGF, His Tag (Cat. No. HGF-H52H3) SDS-PAGE gel

Human HGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human HGF, His Tag (Cat. No. ) MALS images

The purity of Human HGF, His Tag (Cat. No. HGF-H52H3) was more than 90% and the molecular weight of this protein is around 95-105 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human HGF, His TagHuman HGF, His Tag (Cat. No. HGF-H52H3) ELISA bioactivity

Immobilized Human HGF, His Tag at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag (Cat. No. MET-H5256) with a linear range of 1-39 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human HGF R Protein, Fc Tag (Cat. No. MET-H5256) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF Protein, His Tag (Cat. No. HGF-H52H3) with an affinity constant of 75.2 pM as determined in a SPR assay (Biacore 8K) (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human HGF R, Fc Tag (Cat. No. MET-H5256) on Protein A Biosensor, can bind Human HGF, His Tag (Cat. No. HGF-H52H3) with an affinity constant of 3.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. In addition, HGF has been implicated in a variety of cancers, including of the lungs, pancreas, thyroid, colon, and breast.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $400.00

Price(USD) : $2600.00

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