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Your Position: Home > CKP > CD47 > CD7-H82F8

Biotinylated Human CD47 Protein, Fc (L234A, L235A, P329G),Avitag™

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Biotinylated Human CD47, Fc (L234A, L235A, P329G),Avitag (CD7-H82F8) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Online(Gln 19 - Pro 139) Q08722-3

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 41.9 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD47, Fc (L234A, L235A, P329G),Avitag (Cat. No. CD7-H82F8) SDS-PAGE gel

Biotinylated Human CD47, Fc (L234A, L235A, P329G),Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD47, Fc (L234A, L235A, P329G),AvitagBiotinylated Human CD47, Fc (L234A, L235A, P329G),Avitag (Cat. No. CD7-H82F8) ELISA bioactivity

Immobilized Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc (L234A, L235A, P329G),Avitag (Cat. No. CD7-H82F8) with a linear range of 2-39 ng/mL (QC tested).

Biotinylated Human CD47, Fc (L234A, L235A, P329G),AvitagBiotinylated Human CD47, Fc (L234A, L235A, P329G),Avitag (Cat. No. CD7-H82F8) ELISA bioactivity

Immobilized Monoclonal Anti-Human CD47 Antibody, Human IgG4 at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc (L234A, L235A, P329G),Avitag (Cat. No. CD7-H82F8) with a linear range of 0.2-5 ng/mL (Routinely tested).

  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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