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Your Position: Home > Antibodies > Bevacizumab > Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD)

Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD)

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Bevacizumab F(ab')2.
  • Isotype
    IgG1/kappa
  • Specificity
    Recognizes Bevacizumab specifically, no cross reactivity with other humanized antibodies.
  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (13.5 angstroms) is used in this product to minimize potential steric hindrance.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 1-3 as determined by the HABA assay.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 years under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) SDS-PAGE gel

Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD)Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) ELISA bioactivity

Detection of bevacizumab by bridging ELISA in serum. Immobilized Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-Y10) at 2 μg/mL, added increasing concentrations of bevacizumab (10% human serum) and then added Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.4 μg/mL.

Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD)Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) ELISA bioactivity

Immobilized bevacizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) with a linear range of 0.78-25.5 ng/mL.

Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD)Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) ELISA bioactivity

ELISA analysis shows that the binding of bevacizumab to ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) was inhibited by increasing concentration of Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13). The concentration of bevacizumab used is 4 ng/mL.

  • Background
    A recombinant humanized monoclonal IgG1 antibody that binds to and inhibits the biologic activity of human vascular endothelial growth factor (VEGF). Bevacizumab contains human framework regions and the complementarity-determining regions of a murine antibody that binds to VEGF. Bevacizumab is produced in a Chinese Hamster Ovary mammalian cell expression system in a nutrient medium containing the antibiotic gentamicin and has a molecular weight of approximately 149 kilodaltons.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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