>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Detection of bevacizumab by bridging ELISA in serum. Immobilized Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-Y10) at 2 μg/mL, add increasing concentrations of bevacizumab (10% human serum) and then add Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.4 μg/mL.
Immobilized bevacizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13) with a linear range of 0.78-25.5 ng/mL.
ELISA analysis shows that the binding of bevacizumab to ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) was inhibited by increasing concentration of Biotinylated Anti-Bevacizumab Antibodies (recommended for PK/PD) (Cat. No. BEB-BY13). The concentration of bevacizumab used is 4 ng/mL.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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