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Your Position: Home > Fc Receptor Proteins > Fc gamma RIIA / CD32a > CDA-C52H7

Cynomolgus Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & HPLC verified)

  • Synonym
    CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2
  • Source
    Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (CDA-C52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Pro 208 (Accession # Q8SPW4-1).
    Predicted N-terminus: Gln 28
  • Molecular Characterization
    Online(Gln 28 - Pro 208) Q8SPW4-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.1 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) SDS-PAGE gel

Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC
Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. ) HPLC images

The purity of Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) was greater than 90% as determined by SEC-HPLC.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) on CM5 Chip via Anti-His antibody can bind Rituximab with an affinity constant of 4.18 μM as determined in SPR assay (Biacore T200) (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 3.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Rituximab on FAB2G Biosensor, can bind Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. CDA-C52H7) with an affinity constant of 6.0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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