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Your Position: Home > All Other Proteins > PSMA > PSA-C5247

Cynomolgus PSMA / FOLH1 Protein, His Tag (MALS verified) (active enzyme)

  • Synonym
    FOLH1,PSMA,GIG27,FOLH,NAALAD1,PSM,NAALADase I,GCPII,FGCP
  • Source
    Cynomolgus PSMA, His Tag (PSA-C5247) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # A0A2K5VNZ0-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Lys 44 - Ala 750) A0A2K5VNZ0-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 81.4 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of MES and NaCl with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE
Cynomolgus PSMA, His Tag (Cat. No. PSA-C5247) SDS-PAGE gel

Cynomolgus PSMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus PSMA, His Tag (Cat. No. ) MALS images

The purity of Cynomolgus PSMA, His Tag (Cat. No. PSA-C5247) was more than 85% and the molecular weight of this protein is around 185-220 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus PSMA, His TagCynomolgus PSMA, His Tag (Cat. No. PSA-C5247) ELISA bioactivity

Immobilized Cynomolgus PSMA, His Tag (Cat. No. PSA-C5247) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human PSMA Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Bioactivity

Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >300 pmol/min/μg, as measured under the described conditions (QC tested).

  • Background
    Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (NAALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is stable at pH greater than 6.5. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. FOLH1 / GCP-2 is homodimer and binds 2 zinc ions per subunit, and required for NAALADase activity. The catalytic activity of PSMA involved in releasing of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly – gamma - glutamates. FOLH1 / GCP-2 / PSMA has both folate hydrolase and N – acetylated – alpha – linked - acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. GCP-2 / PSMA involved in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $400.00

Price(USD) : $2600.00

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