This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 83.0 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human C2, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human C2, His Tag (Cat. No. CO2-H52H7) is more than 90% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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