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Your Position: Home > All Other Proteins > Glypican 2 > GP2-H5255

Human Glypican 2 / GPC2 Protein, Fc Tag

  • Synonym
    Glypican 2,GPC2
  • Source
    Human Glypican 2, Fc Tag (GP2-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ser 553 (Accession # Q8N158-1).
  • Molecular Characterization
    Online(Ser 24 - Ser 553) Q8N158-1

    This protein carries a human IgG1 Fc tag at the C-terminus. This protein contains a furin cleavage site, 355-RNRR-358, and will be processed into N and C-terminal fragment with calculated MW of 37.3 kDa and 47.3 kDa respectively. The N and C‑terminal fragment remain associated in a covalent complex under non-reducing conditions. The protein migrates as 35 kDa and 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Glypican 2, Fc Tag (Cat. No. GP2-H5255) SDS-PAGE gel

Human Glypican 2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human Glypican 2, Fc Tag (Cat. No. GP2-H5255) on Protein A Biosensor, can bind Human FGF basic, Tag Free (Cat. No. BFF-H4117) with an affinity constant of 0.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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