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Your Position: Home > Immune Checkpoint Proteins > CD27 Ligand > CDL-H5266

Human CD27 Ligand / CD70 Protein, Fc Tag (active trimer) (MALS verified)

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (CDL-H5266) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1) trimer Design.
    Predicted N-terminus: Pro
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 74.1 kDa. The protein migrates as 130-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5266) SDS-PAGE gel

Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. ) MALS images

The purity of Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5266) was more than 85% in HP-SEC, and around 160-190 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD27 Ligand, Fc Tag (active trimer) (MALS verified)Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5266) ELISA bioactivity

Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5266) with a linear range of 0.2-8 ng/mL (QC tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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