This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 49.9 kDa. The protein migrates as 45-55 kDa under non-reducing (NR) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD40 Ligand, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) was more than 85% and the molecular weight of this protein is around 51-69 kDa verified by SEC-MALS.
Loaded Human CD40, Fc Tag (Cat. No. CD0-H5253) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) with an affinity constant of 1.58 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) stimulates secretion of IL-6 by human B cells. The ED50 for this effect is 18.76-19.45 ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4 (Routinely tested).
Neutralization assay shows that the cytokine secretion effect of Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) was inhibited by increasing concentration of anti-CD40L mAb. The concentration of CD40L used is 100 ng/mL. The EC50 is 0.215 μg/mL (Routinely tested).
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