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Your Position: Home > Immune Checkpoint Proteins > 4-1BB Ligand > 41L-H52D4

Human 4-1BB Ligand / TNFSF9 (71-254) Protein, His,Flag Tag (active trimer) (MALS verified)

  • Synonym
    4-1BB Ligand,TNFSF9,CD137L
  • Source
    Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (41L-H52D4) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1) trimer Design.
    Predicted N-terminus: His
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 62.8 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) SDS-PAGE gel

Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. ) MALS images

The purity of Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) was more than 85% in HP-SEC, and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified)Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) ELISA bioactivity

Immobilized Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) at 2 μg/mL (100 μL/well) can bind Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) with a linear range of 0.2-3 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse 4-1BB, Fc Tag (Cat. No. 41B-M5258) on Protein A Biosensor, can bind Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) with an affinity constant of 18.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.20-0.44 μg/mL (Routinely tested).

  • Background
    Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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