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Your Position: Home > Immune Checkpoint Proteins > BTLA > BTA-H5258

Human BTLA (31-150) Protein, Mouse IgG2a Fc Tag

  • Synonym
    BTLA,CD272
  • Source
    Human BTLA (31-150), Mouse IgG2a Fc Tag (BTA-H5258) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).
    Predicted N-terminus: Lys 31
  • Molecular Characterization
    Online(Lys 31 - Ser 150) AAP44003.1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 40.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 100 mM Glycine, 150 mM NaCl, Arginine, pH3.2. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BTLA (31-150), Mouse IgG2a Fc Tag (Cat. No. BTA-H5258) SDS-PAGE gel

Human BTLA (31-150), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human BTLA (31-150), Mouse IgG2a Fc TagHuman BTLA (31-150), Mouse IgG2a Fc Tag (Cat. No. BTA-H5258) ELISA bioactivity

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human BTLA (31-150), Mouse IgG2a Fc Tag (Cat. No. BTA-H5258) with a linear range of 10-156 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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