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Your Position: Home > Immune Checkpoint Proteins > Nectin-4 > NE4-H5255

Human Nectin-4 Protein, Fc Tag

  • Synonym
    NECTIN4,LNIR, PRR4, PVRL4
  • Source
    Human Nectin-4, Fc Tag (NE4-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).
    Predicted N-terminus: Gly 32
  • Molecular Characterization
    Online(Gly 32 - Ser 349) Q96NY8-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.5 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) SDS-PAGE gel

Human Nectin-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Nectin-4, Fc TagHuman Nectin-4, Fc Tag (Cat. No. NE4-H5255) ELISA bioactivity

Immobilized Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with a linear range of 0.313-5 μg/mL (QC tested).

Human Nectin-4, Fc TagHuman Nectin-4, Fc Tag (Cat. No. NE4-H5255) ELISA bioactivity

Immobilized Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Nectin-4 Antibody with a linear range of 0.2-3 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 0.263 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human Nectin-1, His Tag (Cat. No. PV1-H5223) on HIS1K Biosensor, can bind Human Nectin-4, Fc Tag (Cat. No. NE4-H5255) with an affinity constant of 2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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DMF (Drug Master File)

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