This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 58.3 kDa. The protein migrates as 55-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human LIGHT, His Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) is more than 85% in HP-SEC, and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Immobilized Human HVEM, Fc Tag (Cat. No. HVM-H5258) at 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) with a linear range of 16-63 ng/mL (QC tested).
Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.11-3.87 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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