0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > LIGHT > LIT-H5242

Human LIGHT / TNFSF14 Protein, His Tag (active trimer) (MALS verified)

  • Synonym
    CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
  • Source
    Human LIGHT, His Tag (active trimer) (MALS verified) (LIT-H5242) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1) trimer Design.
    Predicted N-terminus: His
  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 58.3 kDa. The protein migrates as 55-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) SDS-PAGE gel

Human LIGHT, His Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. ) MALS images

The purity of Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) was more than 85% in HP-SEC, and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human LIGHT, His Tag (active trimer) (MALS verified)Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) ELISA bioactivity

Immobilized Human HVEM, Fc Tag (Cat. No. HVM-H5258) at 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) with a linear range of 16-63 ng/mL (QC tested).

Human LIGHT, His Tag (active trimer) (MALS verified)Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) ELISA bioactivity

Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) with a linear range of 8-63 ng/mL (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.11-3.87 ng/mL (Routinely tested).

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop