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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 109.2 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rabbit LDL R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human PCSK9 Propeptide & Rabbit Mature PCSK9 (154-686), His Tag at 2 μg/mL (100 μL/well) can bind Rabbit LDL R, Fc Tag (Cat. No. LDR-R5255) with a linear range of 2-78 ng/mL (QC tested).
Loaded Rabbit LDL R, Fc Tag (Cat. No. LDR-R5255) on Protein A Biosensor, can bind Human Apolipoprotein E, His Tag (Cat. No. APE-H5246) with an affinity constant of 56.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Rabbit LDL R, Fc Tag (Cat. No. LDR-R5255) on Protein A Biosensor, can bind Human Apolipoprotein E, His Tag (Cat. No. APE-5256) with an affinity constant of 38.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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