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Your Position: Home > Immune Checkpoint Proteins > LIGHT > LIT-H5269

Human LIGHT / TNFSF14 Protein, Fc Tag (active trimer) (MALS verified)

  • Synonym
    CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
  • Source
    Human LIGHT, Fc Tag (active trimer) (MALS verified) (LIT-H5269) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1) trimer Design.
    Predicted N-terminus: Pro
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 82.6 kDa. The protein migrates as 116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) SDS-PAGE gel

Human LIGHT, Fc Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. ) MALS images

The purity of Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) was more than 90% in HP-SEC, and the molecular weight of this protein is around 180-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human LIGHT, Fc Tag (active trimer) (MALS verified)Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) ELISA bioactivity

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) with a linear range of 0.2-4 ng/mL (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.29-2.38 ng/mL (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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DMF (Drug Master File)

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