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Your Position: Home > Viral Proteins > S protein > SPN-C52H4

SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag

  • Synonym
    S protein,Spike glycoprotein,S glycoprotein,COVID-19
  • Source
    SARS-CoV-2 S protein (R683A, R685A), His Tag (SPN-C52H4) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A, R685A)).
    Predicted N-terminus: Val 16
  • Molecular Characterization
    Online(Val 16 - Pro 1213) QHD43416.1 (R683A, R685A)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 134.6 kDa. The protein migrates as 170-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) SDS-PAGE gel

SARS-CoV-2 S protein (R683A, R685A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
SARS-CoV-2 S protein (R683A, R685A), His TagSARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) ELISA bioactivity

Immobilized SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 1-8 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 61.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) captured on CM5 Chip via anti-His antibody can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with an affinity constant of 2.91 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein (R683A, R685A), His Tag (Cat. No. SPN-C52H4) with an affinity constant of 22.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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