This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS S1 protein, His Tag (Cat. No. S1N-S52H5) is more than 85% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Immobilized SARS S1 protein, His Tag (Cat. No. S1N-S52H5) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-8 ng/mL (QC tested).
Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS S1 protein, His Tag (Cat. No. S1N-S52H5) with an affinity constant of 36.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2
Authors: Cho, Byeong Gwan et al.
Journal: Journal of proteome research 2021
Application: Binding Assay
Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19
Authors: Tosif S, Neeland MR, Sutton P, et al.
Journal: Nature communications 2020
Application: ELISA & FACS
Antibody-like proteins that capture and neutralize SARS-CoV-2
Authors: Kondo T, Iwatani Y, Matsuoka K, et al.
Journal: Science advances 2020
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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