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Your Position: Home > Viral Proteins > S protein > SPN-C52H8

SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, active trimer (MALS verified)

  • Synonym
    S protein,Spike glycoprotein,S glycoprotein,COVID-19
  • Source
    SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (SPN-C52H8) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A, R685A)) trimer Design.
    Predicted N-terminus: Val 16
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-230 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) SDS-PAGE gel

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. ) MALS images

The purity of SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) was more than 80% and around 550-660 kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimerSARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) ELISA bioactivity

Immobilized Biotinylated Human ACE2, Fc,Avitag (Cat. No. AC2-H82F9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with a linear range of 10-40 ng/mL (QC tested).

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimerSARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) ELISA bioactivity

Immobilized SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) with a linear range of 4-63 ng/mL (Routinely tested).

SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimerSARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) ELISA bioactivity

Immobilized SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) ) with a linear range of 0.2441-7.8125 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human ACE2, Fc Tag (Cat. No. AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

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