This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-230 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) was more than 80% and around 550-660 kDa verified by SEC-MALS.
Immobilized Biotinylated Human ACE2, Fc,Avitag (Cat. No. AC2-H82F9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with a linear range of 10-40 ng/mL (QC tested).
Human ACE2, Fc Tag (Cat. No. AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $450.00
Price(USD) : $2800.00
High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed SARS-CoV-2 antigens specifically designed and optimized for serological test kits which are customized formulation available, capable of supplying in bulk order and functionality confirmed.
The affinity constant of SARS-CoV-2 S protein (active trimer) binding to human ACE2 protein was 35.3 nM as verified by SPR.
Binding signal of biotinylated human ACE2 and SARS-CoV-2 S protein RBD was inhibited by SARS-CoV-2 inhibitor with the IC50 10.69 nM.
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