|Items||Size （2mg）||Size（5mg X 2）|
|Particle size||2 μm||2 μm|
|Physical appearance||Powder mixture||Powder mixture|
|Amount of Coupled Protein||>318 pmol (25 μg) Spike S1/mg Beads||>318 pmol (25 μg) Spike S1/mg Beads|
|Binding Capacity||>40 μg anti-SARS2-CoV-2 Spike S1 antibody/mg beads; >20 μg ACE2 protein/mg beads||>40 μg anti-SARS2-CoV-2 S1 antibody/mg beads; >20 μg ACE2 protein/mg beads|
|Formulation||PBS, pH7.4, with 10% Trehalose||PBS, pH7.4, with 10% Trehalose|
|Reconstitution||2 mL ultrapure water (1 mg beads/mL)||5 mL ultrapure water (1 mg beads/mL)|
Upon receipt, please store the lyophilized beads at -20℃ for long term storage. The shelf life is 1 year at -20℃ in lyophilized form.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 1.003 μg/mL (QC tested).
Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.025 μg/mL (QC tested).
The binding curves between SARS-CoV-2 S1 pre-coupling magnetic beads (Cat. No. MBS-K001) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
Price(USD) : $800.00
Price(USD) : $2500.00
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