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Your Position: Home > All Other Proteins > Streptavidin > STN-N5116

Recombinant Streptavidin

  • Synonym
    Streptavidin,SA
  • Source
    Recombinant Streptavidin (STN-N5116) is expressed from E.coli cells.

  • Application
    ELISA assay

  • Formulation

    Lyophilized from 0.22 μm filtered solution in NaCl, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 2 years in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Recombinant StreptavidinRecombinant Streptavidin (Cat. No. STN-N5116) ELISA bioactivity

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested)

Recombinant StreptavidinRecombinant Streptavidin (Cat. No. STN-N5116) ELISA bioactivity

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116)  precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

  • Background
    Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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