COA
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The biotinylated EpCAM protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-EpCAM antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This EpCAM coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
The conjugation was achieved by means of the binding between streptavidin and biotin. Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L. Thus, the binding of streptavidin and biotin is irreversible. Our EpCAM pre-coupled beads could capture anything binding to EpCAM, and make the following testing easy, such as immunocapture, biopanning and flow cytometry.
Application Method:
a) Reconstitute the Beads following the COA. Wash and re-suspended the beads to a certain concentration by adding your dilution buffer.
b) Add the prepared beads to your samples.
c) Beads can be separated from your samples afterwards using a magnetic plate.