This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 24.7 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE).
This protein is used together with nanodisc protein as isotype control.
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.
The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human MSP1D1, His Tag (Nanodisc) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Human MSP1D1, His Tag (Nanodisc) (Cat. No. APO-H51H3), washed and then followed by PE anti-His antibody and analyzed with FACS. PE anti-His antibody was used as negative control (QC tested).
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