Human MSP1D1, His Tag (nanodisc) (APO-H51H3) is an empty nanodisc and assembles from a mixture of phospholipid micelles and membrane scaffold protein(MSP1D1).
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 24.7 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE).
This protein is used together with CD20 nanodisc protein (CD0-H52H1) as isotype control.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human MSP1D1, His Tag (Nanodisc) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Human MSP1D1, His Tag (Nanodisc) (Cat. No. APO-H51H3), washed and then followed by PE anti-His antibody and analyzed with FACS. PE anti-His antibody was used as negative control (QC tested).
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