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Human MSP1D1 Protein, His Tag (Nanodisc)

  • Synonym
  • Source
    Human MSP1D1, His Tag (Nanodisc) (APO-H51H3) is expressed from E.coli cells. It contains AA Gly 1- Gln 211.
    Predicted N-terminus: His
  • Molecular Characterization

    Human MSP1D1, His Tag (nanodisc) (APO-H51H3) is an empty nanodisc and assembles from a mixture of phospholipid micelles and membrane scaffold protein(MSP1D1).
    This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 24.7 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE).
    This protein is used together with CD20 nanodisc protein (CD0-H52H1) as isotype control.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human MSP1D1, His Tag (Nanodisc) (Cat. No. APO-H51H3) SDS-PAGE gel

Human MSP1D1, His Tag (Nanodisc) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

FACS assay shows that recombinant Biotinylated FACS

2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Human MSP1D1, His Tag (Nanodisc) (Cat. No. APO-H51H3), washed and then followed by PE anti-His antibody and analyzed with FACS. PE anti-His antibody was used as negative control (QC tested).

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