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Your Position: Home > Antibodies > Rituximab > RIB-FY35c

FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Rituximab F(ab')2.
  • Isotype
    IgG1/kappa
  • Specificity
    Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.
  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1-3.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (Cat. No. RIB-FY35c) SDS-PAGE gel

FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (Cat. No. RIB-FY35c) ELISA bioactivity

Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (Cat. No. RIB-FY35c) with a linear range of 0.04-0.6 μg/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-CD20 (Rituximab) CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Anti-Rituximab Antibodies, Mouse IgG1 (Cat. No. RIB-FY35c) and isotype control antibody respectively. FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $319.00

Price(USD) : $1980.00

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