This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 138.0 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
SARS-CoV-2 S protein, His Tag, Super stable trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) is more than 90% and the molecular weight of this protein is around 480-550 kDa verified by SEC-MALS.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 1-39 ng/mL (QC tested).
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with an affinity constant of 40.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Authors: Allegra Peletta, Eakachai Prompetchara, et al.
Journal: Vaccines 2021
Authors: Wang W, Li Q, Wu J, et al.
Journal: Emerg Microbes Infect 2021
Authors: Zupancic JM, Desai AA, Schardt JS, et al.
Journal: Cell chemical biology 2021
Application: Binding Assay
Authors: Charvet B, Mazelier M, Brunel J, et al.
Journal: Research Square 2021
Application: Cell-based assay
Authors: Zhou D, Chan JF, Zhou B, et al.
Journal: Cell Host Microbe 2021
Authors: Ozono S, Zhang Y, Ode H, et al.
Journal: Nature communications 2021
Authors: Kazushige Yokoyama, Akane Ichiki
Journal: Colloid and Interface Science Communications 2020
Authors: Wei C, Wan L, Yan Q, et al.
Journal: Nature metabolism 2020
Application: SPR & FACS & Confocal microscopy
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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