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Your Position: Home > Viral Proteins > S protein > SPN-C52H9
  • Synonym
    S protein,Spike glycoprotein,S glycoprotein,COVID-19
  • Source
    SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H9) is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
    Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
    Predicted N-terminus: Val 16
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 138.0 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (MALS & NS-EM verified) (Cat. No. SPN-C52H9) SDS-PAGE gel

SARS-CoV-2 S protein, His Tag, Super stable trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (MALS & NS-EM verified) (Cat. No. ) MALS images

The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) was more than 90% and the molecular weight of this protein is around 520-620 kDa verified by SEC-MALS.

Negative Staining Electron Microscopy

The well-folded trimeric particles of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) was verified by negative stain electron micrography. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature.

Bioactivity-ELISA
SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (MALS & NS-EM verified)SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (MALS & NS-EM verified) (Cat. No. SPN-C52H9) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 1-39 ng/mL (QC tested).

SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (MALS & NS-EM verified)SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (MALS & NS-EM verified) (Cat. No. SPN-C52H9) ELISA bioactivity

Immobilized Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.3-10 ng/mL (Routinely tested).

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Price(USD) : $450.00

Price(USD) : $2800.00

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