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Your Position: Home > All Other Proteins > RNF43 > RN3-H5256

Human RNF43 Protein, Fc Tag (MALS verified)

  • Synonym
    RING finger protein 43,RING-type E3 ubiquitin transferase RNF43
  • Source
    Human RNF43, Fc Tag (RN3-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 44 - Tyr 197 (Accession # Q68DV7-1).
    Predicted N-terminus: Gln 44
  • Molecular Characterization
    Online(Gln 44 - Tyr 197) Q68DV7-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human RNF43, Fc Tag (Cat. No. RN3-H5256) SDS-PAGE gel

Human RNF43, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human RNF43, Fc Tag (Cat. No. ) MALS images

The purity of Human RNF43, Fc Tag (Cat. No. RN3-H5256) was more than 85% and the molecular weight of this protein is around 100-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human RNF43, Fc TagHuman RNF43, Fc Tag (Cat. No. RN3-H5256) ELISA bioactivity

Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 2-20 ng/mL (QC tested).

Human RNF43, Fc TagHuman RNF43, Fc Tag (Cat. No. RN3-H5256) ELISA bioactivity

Immobilized Human R-Spondin 3 (22-146), His Tag (Cat. No. RS6-H5222) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.3-2 ng/mL (Routinely tested).

  • Background
    E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification (By similarity)
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $2310.00

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