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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 75.9 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-17 RC, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) is more than 90% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.
Immobilized Human IL17A, Tag Free (Cat. No. ILA-H5219) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 5-160 ng/mL (QC tested).
Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 0.02-0.25 μg/mL (Routinely tested).
Human IL-17A Protein, Tag Free immobilized on CM5 Chip can Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 72.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Biotinylated Human IL-17A Protein, His,Avitag™ (Cat. No. ILA-H82Q1) captured on Biotin CAP - Series S sensor Chip can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 40.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Human IL17A, His,Avitag (Cat. No. ILA-H82Q1) on SA Biosensor, can bind uman IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 13.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 92.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL-17F, His Tag (Cat. No. ILF-H5244) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 100 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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