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Your Position: Home > Cytokines > IL-17 RA & IL-17 RC > ILC-H5257

Human IL-17 RA & IL-17 RC Heterodimer Protein, Fc Tag&Fc Tag

  • Synonym
    IL-17 RA & IL-17 RC,IL-17RA & IL-17RC,CD217 & IL-17 RC
  • Source
    Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (ILC-H5257) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (IL-17RA) & Leu 21 - His 465 (IL-17RC) (Accession # Q96F46-1(IL-17RA) & NP_703190.1(IL-17RC)).
    Predicted N-terminus: Leu 33 (IL-17RA) & Leu 21 (IL-17RC)
  • Molecular Characterization
    Online(Leu 33 - Trp 320 (IL-17RA) & Leu 21 - His 465 (IL-17RC)) Q96F46-1 (IL-17RA) & NP_703190.1 (IL-17RC)

    Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag is produced by co-expression of IL-17RA and IL-17RC, has a calculated MW of 60.0 kDa (IL-17RA) & 75.7 kDa (IL-17RC). Subunit IL-17RA is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-17RC is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 80-90 kDa and 100-110 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20 to -70°C for 12 months in lyophilized state from date of receipt;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) SDS-PAGE gel

Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human IL17RA & IL17RC Protein, Fc Tag&Fc TagHuman IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) ELISA bioactivity

Immobilized Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) at 2 μg/mL (100 μL/well) can bind Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 1-20 ng/mL (QC tested).

Human IL17RA & IL17RC Protein, Fc Tag&Fc TagHuman IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) ELISA bioactivity

Immobilized Human IL17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 0.6-10 ng/mL (Routinely tested).

Human IL17RA & IL17RC Protein, Fc Tag&Fc TagHuman IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) ELISA bioactivity

Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 2 μg/mL (100 μL/well) can bind Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 1-39 ng/mL (Routinely tested).

  • Background
    Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC. Cytokine binding triggers homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways, ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $2310.00

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    Datasheet & Documentation

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    DMF (Drug Master File)

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