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Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag is produced by co-expression of IL-17RA and IL-17RC, has a calculated MW of 60.0 kDa (IL-17RA) & 75.7 kDa (IL-17RC). Subunit IL-17RA is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-17RC is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 80-90 kDa and 100-110 kDa due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) is more than 90% and the molecular weight of this protein is around 180-210 kDa verified by SEC-MALS.
Immobilized Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag at 2 μg/mL (100 μL/well) can bind Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 1-20 ng/mL (QC tested).
Immobilized Human IL17A, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 0.6-10 ng/mL (Routinely tested).
Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 2 μg/mL (100 μL/well) can bind Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) with a linear range of 1-39 ng/mL (Routinely tested).
Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) captured on CM5 chip via anti-human IgG Fc antibody can bind Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) with an affinity constant of 50.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) on Protein A Biosensor, can bind Human IL17A, Tag Free with an affinity constant of 0.13 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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