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Your Position: Home > All Other Proteins > CCR5 > CC5-H52D1

Human CCR5 Protein, Flag,His Tag

  • Synonym
    CCR5,CMKBR5,CD195,CC-CKR-5,CHEMR13
  • Source
    Human CCR5, Flag,His Tag (CC5-H52D1) is expressed from human 293 cells (HEK293). It contains AA Asp 2 - Arg 223 & Glu 227 - Arg 319 (Accession # P51681-1(C58Y,G163N,A233D,K303E)).
    Predicted N-terminus: Asp
  • Molecular Characterization
    Online(Asp 2 - Arg 223 & Glu 227 - Arg 319) P51681-1(C58Y, G163N, A233D, K303E)

    This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 46.0 kDa. The protein migrates as 33-35 kDa and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with Glycerol as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE
Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) SDS-PAGE gel

Human CCR5, Flag,His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
Human CCR5, Flag,His TagHuman CCR5, Flag,His Tag (Cat. No. CC5-H52D1) ELISA bioactivity

Immobilized Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) at 10 μg/mL (100 μL/well) can bind Purified NA/LE Mouse Anti-Human CD195 (Clone: 2D7) with a linear range of 0.078-2.5 μg/mL (QC tested).

  • Background
    Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation.5 Publications (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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