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Your Position: Home > All Other Proteins > CCR5 > CC5-H52D1

Human CCR5 Protein, Flag,His Tag

  • Synonym
    CCR5,CMKBR5,CD195,CC-CKR-5,CHEMR13
  • Source
    Human CCR5, Flag,His Tag (CC5-H52D1) is expressed from human 293 cells (HEK293). It contains AA Asp 2 - Arg 223 & Glu 227 - Arg 319 (Accession # P51681-1(C58Y, G163N, A233D, K303E)).
    Predicted N-terminus: Asp
  • Molecular Characterization
    Online(Asp 2 - Arg 223 & Glu 227 - Arg 319) P51681-1 (C58Y, G163N, A233D, K303E)

    This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 46.0 kDa. The protein migrates as 33-35 kDa and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep CCR5 soluble and active, under no circumastance should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
SDS-PAGE
Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) SDS-PAGE gel

Human CCR5, Flag,His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
Human CCR5, Flag,His TagHuman CCR5, Flag,His Tag (Cat. No. CC5-H52D1) ELISA bioactivity

Immobilized Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) at 10 μg/mL (100 μL/well) can bind Purified NA/LE Mouse Anti-Human CD195 (Clone: 2D7) with a linear range of 0.078-2.5 μg/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) captured on CM5 chip via anti-His antibody can bind Anti-CCR5 antibody (Human IgG1) with an affinity constant of 0.0737 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Purified NA/LE Mouse Anti-Human CD195 (Clone: 2D7) (Mouse IgG2a) captured on CM5 chip via anti-mouse antibodies surface can bind Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) with an affinity constant of 7.51 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).

  • Background
    Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation.5 Publications (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:12 Details
  • Latest Research Phase:Approved

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