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Your Position: Home > Immune Checkpoint Proteins > GITR > GIR-H82E1

Biotinylated Human GITR / TNFRSF18 Protein, His,Avitag™ (MALS verified)

  • Synonym
    AITR,GITR,TNFRSF18,CD357
  • Source
    Biotinylated Human GITR, His,Avitag™ (GIR-H82E1) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    Online(Gln 26 - Glu 161) Q9Y5U5-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 18.1 kDa. The protein migrates as 22-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human GITR, His,Avitag™ (Cat. No. GIR-H82E1) SDS-PAGE gel

Biotinylated Human GITR, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human GITR, His,Avitag™ (Cat. No. ) MALS images

The purity of Biotinylated Human GITR, His,Avitag™(Cat. No. GIR-H82E1) was more than 95% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human GITR, His,Avitag™Biotinylated Human GITR, His,Avitag™ (Cat. No. GIR-H82E1) ELISA bioactivity

Immobilized Human GITR Ligand, Fc Tag (Cat. No. GIL-H5261) at 10 μg/mL (100 μL/well) can bind Biotinylated Human GITR, His,Avitag (Cat. No. GIR-H82E1) with a linear range of 0.2-8 ng/mL (QC tested).

  • Background
    Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $350.00

    Price(USD) : $1380.00

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