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Your Position: Home > Protein > ZNRF3 > ZN3-H52H3

Human ZNRF3 protein, His Tag (MALS verified)

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  • Synonym
    ZNRF3, Zinc,RING finger protein 3, RING-type E3 ubiquitin transferase ZNRF3, RING finger protein 203, KIAA1133, RNF203
  • Source
    Human ZNRF3, His Tag (ZN3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 56 - Met 219 (Accession # Q9ULT6-1).
    Predicted N-terminus: Lys 56
  • Molecular Characterization
    Online(Lys 56 - Met 219) Q9ULT6-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 19.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ZNRF3, His Tag (Cat. No. ZN3-H52H3) SDS-PAGE gel

Human ZNRF3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human ZNRF3, His Tag (Cat. No. ) MALS images

The purity of Human ZNRF3, His Tag (Cat. No. ZN3-H52H3) is more than 90% and the molecular weight of this protein is around 18-27 kDa verified by SEC-MALS.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human ZNRF3, His Tag (Cat. No. ZN3-H52H3) on HISIK Biosensor, can bind Human R-Spondin 3 Protein, Fc Tag with an affinity constant of 16.05 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human R-Spondin 3 Protein, Fc Tag on Protein A Biosensor, can bind Human ZNRF3, His Tag (Cat. No. ZN3-H52H3) with an affinity constant of 10.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    The transmembrane E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) is a negative feedback regulator of Wnt signalling. ZNRF3 is associated with the Wnt receptor complex, and inhibits Wnt signalling by promoting the turnover of frizzled and LRP6 and acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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