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Your Position: Home > All Other Proteins > Siglec-3 > CD3-H5226

Human Siglec-3 / CD33 Protein, His Tag (MALS verified)

  • Synonym
    CD33,SIGLEC3,gp67
  • Source
    Human Siglec-3, His Tag (CD3-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
    Predicted N-terminus: Asp 18
  • Molecular Characterization
    Online(Asp 18 - His 259) AAH28152.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.6 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-3, His Tag (Cat. No. CD3-H5226) SDS-PAGE gel

Human Siglec-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Siglec-3, His Tag (Cat. No. ) MALS images

The purity of Human Siglec-3, His Tag (Cat. No. CD3-H5226) was more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Siglec-3, His TagHuman Siglec-3, His Tag (Cat. No. CD3-H5226) ELISA bioactivity

Immobilized Anti-Human CD33 MAb, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human Siglec-3, His Tag (Cat. No. CD3-H5226) with a linear range of 0.1-2 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Anti-Human CD33 MAb, Mouse IgG1 on AMC Biosensor, can bind Human Siglec-3, His Tag (Cat. No. CD3-H5226) with an affinity constant of 1.86 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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