This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.9 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-1 Rrp2 (C154S, C262S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-1 Rrp2 (C154S, C262S), His Tag(Cat. No. IL2-H52H4) is more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
Immobilized Human IL-1 Rrp2 (C154S, C262S), His Tag (Cat. No. IL2-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-Human IL-1 Rrp2 MAb, Human IgG with a linear range of 0.2-3 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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