This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS S protein (R667A, K968P, V969P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS S protein (R667A, K968P, V969P), His Tag(Cat. No. SPN-S52H6) is more than 90% and the molecular weight of this protein is around 530-570 kDa verified by SEC-MALS, indicating that this protein is in trimeric form.
Immobilized SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).
Immobilized Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind SARS S protein (R667A, K968P, V969P), His Tag (Cat. No. SPN-S52H6) with a linear range of 5-78 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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