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Your Position: Home > All Other Proteins > NKp46 > NC1-C82E5

Biotinylated Cynomolgus NKp46 / NCR1 / CD335 Protein, His,Avitag™ (MALS verified)

  • Synonym
    NCR1,LY94,CD335,NK-p46,hNKp46
  • Source
    Biotinylated Cynomolgus NKp46, His,Avitag (NC1-C82E5) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Arg 257 (Accession # Q95JB9-1).
    Predicted N-terminus: Pro 22
  • Molecular Characterization
    Online(Pro 22 - Arg 257) Q95JB9-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 30.3 kDa. The protein migrates as 35-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus NKp46, His,Avitag (Cat. No. NC1-C82E5) SDS-PAGE gel

Biotinylated Cynomolgus NKp46, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Cynomolgus NKp46, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Cynomolgus NKp46, His,Avitag(Cat. No. NC1-C82E5) was more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Cynomolgus NKp46, His,AvitagBiotinylated Cynomolgus NKp46, His,Avitag (Cat. No. NC1-C82E5) ELISA bioactivity

Immobilized Biotinylated Cynomolgus NKp46, His,Avitag (Cat. No. NC1-C82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-NKp46 Antibody, Chimeric mAb, Human IgG4 with a linear range of 0.1-2 ng/mL (QC tested).

Biotinylated Cynomolgus NKp46, His,AvitagBiotinylated Cynomolgus NKp46, His,Avitag (Cat. No. NC1-C82E5) ELISA bioactivity

Immobilized Anti-NKp46 Antibody, Chimeric mAb, Human IgG4 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus NKp46, His,Avitag (Cat. No. NC1-C82E5) with a linear range of 0.2-3 ng/mL (Routinely tested).

  • Background
    Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1 contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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