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Your Position: Home > Protein > PVRIG > PVG-H82F5

Biotinylated Human PVRIG Protein, Mouse IgG2a Fc,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

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  • Synonym
    C7orf15,C7orf15MGC138295,CD112R,MGC104322,MGC138297,MGC2463,PVRIG,CD112 receptor
  • Source
    Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (PVG-H82F5) is expressed from human 293 cells (HEK293). It contains AA Thr 41 - Asp 171 (Accession # Q6DKI7-1).
  • Molecular Characterization
    Online(Thr 41 - Asp 171) Q6DKI7-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 42.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) SDS-PAGE gel

Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) is more than 90% and the molecular weight of this protein is around 95-105 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human PVRIG, Mouse IgG2a Fc,AvitagBiotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) ELISA bioactivity

Immobilized Human Nectin-2, His Tag (Cat. No. PV2-H52E2) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) with a linear range of 0.02-0.313 μg/mL (QC tested).

Biotinylated Human PVRIG, Mouse IgG2a Fc,AvitagBiotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) ELISA bioactivity

Immobilized Human Nectin-2, Fc Tag (Cat. No. PV2-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) with a linear range of 19.5-156 ng/mL (Routinely tested).

Biotinylated Human PVRIG, Mouse IgG2a Fc,AvitagBiotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) ELISA bioactivity

Immobilized Biotinylated Human PVRIG, Mouse IgG2a Fc,Avitag (Cat. No. PVG-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Nectin-2, Fc Tag (Cat. No. PV2-H5253) with a linear range of 0.6-2 ng/mL (Routinely tested).

  • Background
    Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The CD112R gene encodes a putative single transmembrane protein, which is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. Notably, the intracellular domain of phatases. The extracellular domain sequence of human and mouse CD112R have 65.3% similarity. CD112R may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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