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Product Details
Synonyms
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Source
SARS-CoV-2 S protein RBD (K417N,E484K,N501Y), Fc Tag (SPD-C5256) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417N, E484K, N501Y)). The K417N, E484K, N501Y mutations were identified in the SARS-CoV-2 Beta variant (Pango lineage: B.1.351; other names: 20H/501Y.V2).
Predicted N-terminus: Arg 319
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Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) is more than 90% and the molecular weight of this protein is around 110-130 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with a linear range of 39-156 ng/mL (QC tested).
Protocol
Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) with a linear range of 0.1-0.8 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5256) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.03 nM as determined in BLI assay (ForteBio Octet Red96e)(Routinely tested).
Protocol
Customer Reviews Writing Reviews
C***k2021-09-01
LikesWorking on COVID-19, Acrobiosystems technical service was very quick and precise to answer my question and the customer service department very reactive. The product SPD-C5256 and SPD-C525e were very good for in vitro testing, reproducible and acurate on binding assay with different proteins and antibodies which were able to determin Kds for each one of them.
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